E-ISBN: E 978-81-8487-541-6
Publication Year: Reprint 2014
Binding: Paper Back Dimension: 160mm x 240mm Weight: 415
About the book
RECOMBINANT DNA TECHNIQUES: A Textbook has all the techniques used in the Genetic Engineering like the PCR, Microarray, transfection techniques, Blotting techniques, DNA sequencing, site directed Mutagenesis and protein engineering. Also various aspects of the gene therapy. It also have the good description of the mapping techniques along with the various molecular markers used in the mapping of the genomes like RFLP, RAPD, AFLP etc. DNA chip technology is the most important techniques used for the study of the gene expression and it is the only technique that can analyze the multiple genes at a time. This techniques is very well explained in the book. DNA sequencing by Sangerís Method and maxam and Gilbertís method is also explained by the help of good diagrams. These are the important topics covered in this book.
Table of Contents
Acknowledgement / Preface / Introduction to Recombinant DNA Technology / Enzymes in Genetic Engineering / Nucleic Acid Isolation / Polymerase Chain Reaction / Cloning Vectors / Gene Sequencing / cDNA Synthesis and Cloning / Alternative Strategies of Gene Cloning / Site Directed Mutagenesis and Protein Engineering / Techniques Associated with Cloning / Production of Recombinant Proteins / DNA Chip Technology and Microarray / Genome Mapping / Gene Therapy / Gene Tagging and Gene Knockout Technology / Index.
Undergraduate-Postgraduate Students and Professionals