ISBN: 978-81-8487-443-3 
Publication Year:   2015
Pages:   214
Binding:   Paper Back

PLANT TISSUE CULTURE: Protocols in Plant Biotechnology discusses basic essential techniques/methodologies of plant tissue culture including histological techniques, ex vitro rooting, hardening and acclimatization.. The information on how to analyze the plant tissue culture data through statistical analysis with examples, the recommended setting for taking photographs of tissue culture data through statistical analysis with examples and also the recommended setting for taking photographs of tissue culture material are highlighted. The reproducible protocols for various propagation, crop improvement and cost-reduction techniques is elucidated. The book also provides information on interpretation of the recorded data. Each exercise in this book is profusely illustrated with original photographs. The protocols discussed are original contributions and are reproducible.

Introduction and Essential Techniques: Laboratory Organization / Equipment and Instruments / Sterilization / Tissue Culture Media / Squash Preparation of Callus Tissue / Histological Technique / Ex Vitro Rooting / Hardening and Acclimatization / Statistical Analysis / Photographic methods for Plant Tissue Culture /Propagation Techniques: Selection of Material and Explants / Seed Culture / Micropropagation / Apical Bud Culture / Meristem Culture / Axillary Bud Culture / Adventitious Bud Culture / Induction of Callus / Initiation and Establishment of Cell Suspension Cultures / Organogenesis from Callus / Somatic Embryogenesis / Production of Synthetic Seeds / Regeneration of Plants from Synthetic Seeds / Embryo Culture / Crop Improvement Techniques: Somaclonal Variations / Isolation and Culture Protoplast / Anther and Pollen Culture / Ovary or Ovule Culture / Confirmation of Ploidy Level / Commercial Tissue Culture Technique: Cost Reduction / References / Index.