ISBN: 978-0-7637-4782-4 
Publication Year:   2005
Pages:   204
Binding:   Hard Back

DNA sequencing techniques have evolved rapidly in recent years — largely resulting from the Human Genome Project — but scientists still encounter challenges in sequencing genomes. DNA Sequencing: Optimizing the Process and Analysis was written to help scientists sequence difficult templates. It is also a practical guide to faster and more efficient routine DNA sequencing. This in-depth volume covers important topics in the field, including biochemical and technological advances stemming from the Human Genome Project, proven and newly emerging methods of preparing DNA templates, the effects of widely used laboratory reagents on DNA sequencing, Laboratory Information Management Systems (LIMS), setting up of a GLP/GMP DNA sequencing lab, and the future of DNA sequencing. "... [DNA Sequencing: Optimizing the Process and Analysis offers] fascinating insight into the many and varied approaches to solving problem sequences, to say nothing of the appreciation one should gain that this has been, and remains, a difficult set of problems to solve." -From the Foreword by Elaine R. Mardis

Foreword / Controlled Heat-Denaturation of DNA Plasmids / Effect of Various Reagents on DNA Sequencing / Sequencing of Difficult DNA Templates / New DNA Sequencing Enzymes / Beyond pUC: Vectors for Cloning Unstable DNA / Recombination-Based Cloning / Plasmid Preparation Methods for DNA Sequencing / Optimization of Culture Growth in 96-Deep-Well Plates / Automated DNA Scanners Used in Sequencing Laboratories / Geospiza’s Finch-Server: A Complete Data Management System for DNA Sequencing / DNA Sequencing Database: A Flexible LIMS for DNA Sequencing Analysis / Good Laboratory Practices, Good Manufacturing Procedures, and Quality Assurance in the DNA Sequencing Laboratory / The Future of DNA Sequencing: Towards an Affordable Genome